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WG1 EmergentViruses
WG2 AnalyticalMethods
WG3 DataAnalysis
WG4 StabilityOfViruses
WG2 ID WG2 Deputy Leader name and Institution WG2 Leader name and Institution MAIN MATRIX MATRIX Main Organism Min Max Units Notes SAMPLE TREATMENT CONCENTRATION VIRUS EXTRACTION RNA EXTRACTION DETECTION Country Lab Code City PCR ID Reference ID Deliverable Institutions ID Picture Remarks Picture Th
View 1 WATER Drinking / treated water 2 2 L In case of Chlorine residues, 1,5 L of water neutr More ... Filtration positively charged membrane Zetapor Filter membrane incubated in 3 mL of NucliSens More ... Magnetic silica (Nuclisens EasyMag, BioMérieux) France G Paris 473 68 Image from DB Image from DB
View 2 WATER Drinking / treated water 2 2 L In case of Chlorine residues, 1,5 L of water neutr More ... Filtration positively charged membrane Zetapor Filter membrane incubated in 3 mL of NucliSens More ... Magnetic silica (Nuclisens EasyMag, BioMérieux) France G Paris 1,051 68 Image from DB Image from DB
View 3 WATER Drinking / treated water 10 L Acidification before filtering Filtration Cellulose nitre membrane, beef extract protein precipitation & centrifugatio More ... Spin columns (Qiagen) OR magnetic silica (Nuclisen More ... Great Britain I London Image from DB Image from DB
View 4 WATER Drinking / treated water 100 L - Ultrafiltration 1t stage Ultrafiltration 2nd stage Spin columns (Qiagen) Italy K Pisa 5 Image from DB Image from DB
View 5 WATER Drinking / treated water 1 L Filtration Ultrafiltration - Magnetic silica (Nuclisens MiniMag, BioMérieux) Norway N Oslo 1,003 37 Image from DB Image from DB
View 6 WATER Drinking / treated water 5 10 L ”buffering” with 1/10 (v/v) Phospahte 0,5M ph=7 Using CIM® chromatographic supports - Trizol or Quiagen viral RNA kit Slovenia 1 O 42 Image from DB Image from DB
View 7 WATER Drinking / treated water 2 4 L - Ultrafiltration, ultracentrifugation - TRIzol OR Qiagen spin columns Slovenia P Ljubliana 1,271 43 Image from DB Image from DB
View 8 WATER Drinking / treated water 1 2,500 L river water Pre-acidification to PH 3.5. Glass wool wash with More ... Filtration Glass wool, membrane Elution with Glycine buffer-beef extract. Nucleospin RNA virus F (Macherey More ... Spain R Barcelona 2 39 3 Image from DB Image from DB
View 9 WATER Drinking / treated water 500 1,000 L - Filtration Positively charged membrane - Magnetic silica (Nuclisens, BioMérieux) (OR Boom?) Spain Q Barcelona 1 918 27 Image from DB Image from DB
View 10 WATER Drinking / treated water L unknown ultrafiltration Precipitation PEG - Boom and magnetic silica (Nuclisens MiniMag, BioM More ... France F Nantes 167 30 Image from DB Image from DB
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